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KMID : 1149220150230020189
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Korean Journal of Oriental Medical Prescription
2015 Volume.23 No. 2 p.189 ~ p.198
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Mitochondria protection of Sparganii Rhizoma against oxidative stress in heptocytes
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Seo Hye-Lim
Lee Ju-Hee
Jagn Mi-Hee
Kwon Young-Won
Cho Il-Je
Kim Kwang-Joong
Park Sook-Jahr
Kim Sang-Chan
Kim Young-Woo
Byun Sung-Hui
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Abstract
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Objectives : Sparganii Rhizoma is frequently used in traditional herbal medicine for treatment of blood stasis, amenorrhea and functional dyspepsia and has been reported to exhibit anti-oxidant, anti-proliferation and anti-angiogenesis peoperties. In this study, we investigated the cytoprotective effect and underlying mechanism of Sparganii Rhizoma water extract (SRE) against oxidative stress-induced mitochondrial dysfunction and apoptosis in hepatocyte.
Methods : To determine the effects of SRE on oxidative stress, we induced synergistic cytotoxicity by co-treatment of
arachidonic acid (AA) and iron in the HepG2 cell, a human derived hepatocyte cell line.
Results : Treatment of SRE increased relative cell viability and altered the expression levels of apoptosis-related proteins such as Bcl-xL, Bcl-2 and procaspase-3. And SRE also inhibited the mitochondrial dysfunction and excessive reactive oxygen species production induced by AA+iron. In addition, SRE activated of AMP-activated protein kinase (AMPK), a potential target for cytoprotection, by increasing the phosphorylation of AMPKα at Thr-172. Morever, SRE increased phosphorylation of acetyl-CoA carboxylase, a direct downstream target of AMPK.
Conclusion : These results indicated that SRE has the ability to protect against oxidative stress-induced hepatocyte damage, which may be mediated with AMPK pathway.
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KEYWORD
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Sparganii Rhizoma, hepatocyte, mitochondria, oxidative stress, AMPK
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